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Image Search Results
Journal: Stem Cell Research & Therapy
Article Title: Potential effect of amniotic fluid-derived stem cells on hyperoxia-induced pulmonary alveolar injury
doi: 10.1186/s13287-022-02821-3
Figure Lengend Snippet: Effect of stem cell treatment on biochemical markers. A Western blot analyses of IL-6 and RhoA in lung tissue in all groups. β actin was used for normalization. Images of the bands where obtained from the same gel with no cropping in between the bands. Full length blots are included in the Additional file Fig. S1. B , C Representative graphs for relative expression of IL-6 and RhoA respectively. D , E Representative graphs for quantification of MDA and TAC in lung tissue of all experimental groups. Data is represented in mean. n = 3 in all groups. a; significant compared to CG. b: significant compared to HG. c: significant compared to CFM-G. d: significant compared to SC-PG. e: significant compared to SC-TG. Pairwise comparison bet. Each 2 groups using Post Hoc Test (Tukey). Significance at p ≤ 0.05. Error bars represent S.E.M. Stem cell treatment significantly reduced IL-6, RhoA and increased TAC, while stem cell prophylaxis significantly reduced MDA. Abbreviations: CG; control group, HG; Hyperoxia group, CFM-G; Cell-free media-treated group, SC-PG; Stem cells-prophylactic group, SC-TG; Stem cells-treated group, Unt-G Untreated group, IL-6; interleukin 6, RhoA; Ras homolog family member A, MDA; malondialdehyde, TAC; total antioxidant capacity
Article Snippet: The lung tissues were homogenized in 1–2 ml of 5 mM cold potassium phosphate buffer (pH 7.4), centrifuged at 4000 rpm for 15 min at 4 °C and the supernatant was removed and stored at − 80 °C for assay of total
Techniques: Western Blot, Expressing, Comparison, Control